Product Reference
2025
01
Theranostics 2025, Vol. 15, Issue 3
Materials and Methods
RNA sequencing analysis
The gene-level analysis was conducted by ATG
Lifetech Inc. (Republic of Korea). Briefly, total RNA
(10 ng) was extracted from liver tissues using S2 +
Reverse-transcriptase (Cat# LP-T0012, ATG Lifetech
Inc.), and RNA integrity was evaluated by analyzing
the RNA Integrity Number (RIN). Complementary
DNA (cDNA) was subsequently amplified using K1
HiFi PCR Master Mix (Cat# LP-T0013, ATG Lifetech
Inc.). Tn5 transposase was employed to ligate
next-generation sequencing (NGS) adapters to the
cDNA, with only adapter-ligated fragments being
amplified by the NGS Library Prep Kit (LP-T0011,
ATG Lifetech Inc.).